2.10 Life cycle of Dictyostelium discoideum

<strong>2.10</strong> <strong>Life</strong> <strong>cycle</strong> <strong>of</strong> <strong>Dictyostelium</strong> <strong>discoideum</strong>

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2.12 The three cell adhesion molecules <strong>of</strong> <strong>Dictyostelium</strong>

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3.24 Sorting out and reconstruction <strong>of</strong> spatial relationships in aggregates <strong>of</strong>embryonic amphibian cells

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3.27 Hierarchy <strong>of</strong> cell sorting in order <strong>of</strong> decreasing surface tensions

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Fate Maps <strong>of</strong> Different Vertebrate Classes at the Early Gastrula Stage

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Xenopus fate map

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Vital Dye Staining <strong>of</strong> Amphibian Embryos

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Fate Mapping Using a Fluorescent Dye

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Fate Mapping Using a Fluorescent Dye

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Genetic Markers as Cell Lineage Tracers

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Experimental manipulationRemove cells (blastomeres), kill,ablateIsolate blastomeres, or portions <strong>of</strong>early embryosTransplant portions to new sitesOR +/-GENES

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Acetylcholinesterase in the Progeny <strong>of</strong> the Muscle Lineage Blastomeres

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Microsurgery on Tunicate Eggs

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Roux’s Attempt to Demonstrate Mosaic Development

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Driesch’s Demonstration <strong>of</strong> Regulative Development

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Conditional Specification• Regulative vs• Mosaic

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Activin (Or a Closely Related Protein) Is Thought to Be Responsible for ConvertingAnimal Hemisphere Cells into Mesoderm

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2.13 Using antibodies to show location and function <strong>of</strong> a particular protein